Description: Next Generation Sequencing in Forensic Science : A Primer, Paperback by Elkins, Kelly M.; Zeller, Cynthia B., ISBN 1032072040, ISBN-13 9781032072043, Brand New, Free shipping in the US "Next Generation Sequencing in Forensic Science: A Primer addresses Next Generation Sequencing (NGS) specific to its application to forensic science. Th opens with a history of human identity approaches including VNTR, RFLP, STR, and SNP DNA typing. It next discusses the history of sequencing for human DNA typing including: Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. Readers are expected to have as basic understanding of molecular and cellular biology and DNA typing. Chapters present an overview of the forensically-focused AmpliSeq, ForenSeq, Precision ID, PowerSeq and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs run on the MiSeq FGx and Ion Torrent System. Usingthese, that authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of th details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef including creating a sample list, wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained including how to analyze and interpret NGS data and output graphs and charts. Th concludes with the mitochondrial chromosome and mitochondrial DNA (mtDNA) sequencing and SNPs analysis including the issue of heteroplasmy. The final chapters review applications of microbial DNA for forensics, NGS in body fluid analysis, and challenges and considerations for future applications"--
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Book Title: Next Generation Sequencing in Forensic Science : A Primer
Number of Pages: 162 Pages
Publication Name: Next Generation Sequencing in Forensic Science : a Primer
Language: English
Publisher: CRC Press LLC
Subject: Public Health, Life Sciences / Biochemistry, Life Sciences / Genetics & Genomics, Forensic Science
Publication Year: 2021
Item Weight: 11.9 Oz
Type: Textbook
Item Length: 9.2 in
Subject Area: Law, Science, Medical
Author: Cynthia B. Zeller, Kelly M. Elkins
Item Width: 6.1 in
Format: Trade Paperback
